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Related Experiment Video

Updated: Jun 8, 2026

Axonal Transport of Organelles in Motor Neuron Cultures using Microfluidic Chambers System

Axonal Transport of Organelles in Motor Neuron Cultures using Microfluidic Chambers System

Published on: May 5, 2020

Difference Tracker: ImageJ plugins for fully automated analysis of multiple axonal transport parameters.

Simon Andrews¹, Jonathan Gilley, Michael P Coleman

¹Bioinformatics Group, The Babraham Institute, Babraham, Cambridge, UK.

Journal of Neuroscience Methods

|September 28, 2010

Summary

Difference Tracker, a new ImageJ plugin, offers fast, automated analysis of axonal transport. This tool objectively quantifies particle movement in live nerve imaging, aiding disease and therapy research.

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Area of Science:

Neuroscience
Cell Biology
Biophysics

Background:

Axonal transport is crucial for neuronal function and implicated in diseases.
Live imaging of axonal transport in mammalian nerves offers new research avenues.
Manual analysis of axonal transport is time-consuming and subjective.

Purpose of the Study:

To develop a fast, automated, and objective method for analyzing axonal transport.
To provide a free, accessible tool for researchers studying particle movement in axons.

Main Methods:

Development of Difference Tracker, a software combining two ImageJ plugins.
Utilizing Difference Tracker for automated tracking of fluorescently labeled particles.
Validation using artificial simulations and live imaging of mammalian tibial nerve axons.

Main Results:

Difference Tracker accurately tracks particles in simulations.
The software successfully identifies and tracks multiple motile mitochondria in live nerve axons.
Quantitative parameters obtained manually and by Difference Tracker are comparable.

Conclusions:

Difference Tracker is a valuable, free resource for objective and efficient comparative analysis of axonal transport.
The tool facilitates research into disease, aging, and therapeutic effects on neuronal transport.
Potential for broader application in studies requiring quantification of particle movement.