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Expression of active and inactive recombinant soluble trehalase using baculovirus-silkworm expression system and
Minoru Ujita1, Mayumi Yamanaka, Yumiko Maeno
1Laboratory of Biological Chemistry, Department of Applied Biological Chemistry, Faculty of Agriculture, Meijo University, Tempaku-ku, Nagoya 468-8502, Japan. ujita@meijo-u.ac.jp
Abstract:
Silkworm soluble trehalase was expressed as a fusion protein with an N-terminal or C-terminal hexahistidine tag in a baculovirus-silkworm expression system and assayed for enzymatic activity. Only N-terminally tagged trehalase showed a high activity. This study is the first to report in vitro functional expression of recombinant insect soluble trehalase.
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