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Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

Updated: Jun 8, 2026

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Published on: August 2, 2015

Functional protein microarray technology.

Shaohui Hu¹, Zhi Xie, Jiang Qian

¹Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD, USA.

Wiley Interdisciplinary Reviews. Systems Biology and Medicine

|September 28, 2010

Summary

This summary is machine-generated.

Functional protein microarrays are powerful tools for proteomics research. These arrays enable high-throughput studies of biomolecular interactions and post-translational modifications, aiding biomarker discovery and diagnostics.

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Area of Science:

Proteomics
Biotechnology
Systems Biology

Background:

Functional protein microarrays are advanced tools for large-scale biological studies.
They offer high-throughput analysis capabilities crucial for modern research.

Purpose of the Study:

To review the applications of functional protein microarrays in basic proteomics.
To highlight their use in profiling protein post-translational modifications.
To discuss their potential in biomarker identification and clinical diagnostics.

Main Methods:

Development of various assays to probe binding activities to biomolecules (proteins, DNA, RNA, small molecules, glycans).
Profiling of protein post-translational modifications such as phosphorylation, ubiquitylation, acetylation, and nitrosylation.

Main Results:

Demonstrated utility in basic proteomics research for analyzing biomolecular interactions.
Successful application in profiling diverse protein post-translational modifications.
Potential shown for biomarker identification and clinical diagnostic applications.

Conclusions:

Functional protein microarrays are versatile tools for proteomics.
They are increasingly valuable for understanding biological systems and disease.
These arrays are poised to become indispensable in research and diagnostics.