Artificial perineurium to enhance nerve recovery from damage after neurolysis

We have developed a novel biodegradable poly-lactide (PLA) film (honeycomb film) with a micropatterned porous structure on one side. We hypothesized that this film could be used as a substitute for perineurium. We used two types of thin PLA film: honeycomb film and cast film with smooth surfaces on both sides. In a rat extensive internal neurolysis model, the nerve was wrapped with honeycomb film (group H) or cast film (group CA), or left unwrapped (group C). Histological and functional analyses were performed. The honeycomb film closely attached to the nerve surface but did not adhere to surrounding tissues. In contrast, nerves in group C displayed severe adhesion to the neural bed. Mean percent wet muscle weight and motor nerve conduction velocity were significantly higher in group H than in group C. The honeycomb film prevents nerve adhesion and enhances functional recovery after extensive neurolysis.