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Monocarboxylate carrier from bovine heart mitochondria: reconstitution in a functionally active state
1Department of Cellular Biochemistry, Nencki Institute of Experimental Biology, Warszawa, Poland.
Acta Biochimica Polonica
|January 1, 1990
Abstract:
A method has been developed for functional reconstitution of the isolated pyruvate carrier. Optimal conditions were reached after removal of deoxycholate with 27 mg Triton X-100 per gram of moist Amberlite XAD-2 beads and 7 micrograms protein/mg of phospholipid. Uptake of pyruvate into proteoliposomes and sensitivity of the carrier towards inhibitors resemble those in intact mitochondria.