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Interactions of ferrous ions with peroxidized lecithin-liposomes
1Department of Biochemistry and Biophysics, Silesian Medical Academy, Sosnowiec, Poland.
Abstract:
Oxidation of Fe2+ in the presence of peroxidized lecithin-liposomes small unilamellar vesicles suspended in various media (pH = 5.5) is described. Peroxidation of lecithin was induced by UV light and assayed by three methods: estimation of conjugated dienes, accumulation of hydroperoxides and by the thiobarbituric acid test. The oxidation of Fe2+ was accelerated by phosphate and citrate buffers, but in acetate buffer it was dependent on lipid peroxidases concentration and exhibited saturation kinetics.
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