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[An HGPRT defective mouse lung cancer cell line and its biological characteristics.]
1Cancer Center and Cancer Institute of The First University Hospital , West China University of Medical Sciences , Chengdu 610041 , P. R. China.
Background:
To provide antigenic cells suitable for cell fusion to preparing lung cancer vaccine.
Methods:
An HGPRT (hypoxanthine guanidine phosphoribosyl transferase)-defective cell line , ALA9702 , was gradually induced by 8-azaguanine (8-AG) from mouse lung cancer cell line , LA795 . The biological characteristics and carcinogenicity of ALA9702 cells have been appraised both in vivo and in vitro.
Results:
ALA9702 cells have been cultured over one year in medium containing 20mug/ml 8-AG, and maintained their biologic characteristics and carcinogenicity of the original cells , LA795 cells.
Conclusions:
ALA9702 cell line presents as stabile HGPRT-defective mouse lung cancer cell. It will provide useful antigenic cells for establishment of effective specific lung cancer vaccine therapy.
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