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Updated: Jun 8, 2026

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
Published on: December 9, 2013
Fluorescence lidar multicolor imaging of vegetation
Abstract:
Multicolor imaging of vegetation fluorescence following laser excitation is reported for distances of 50 m. A mobile laser-radar system equipped with a Nd:YAG laser transmitter and a 40-cm-diameter telescope was utilized. The laser light was Raman shifted to 397 nm with pulse energies of ˜ 30 mJ. An image-intensified CCD camera with a specially designed split-mirror Cassegrainian telescope was utilized for the simultaneous recording of fluorescence images of leaves and branches in four different spectral bands. Additionally, fluorescence spectra at selected points within the detection area were measured with an image-intensified diode array system. Image processing permits extraction of information related to the physiological status of the vegetation and might prove useful in forest decline research.
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