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Updated: Jun 8, 2026

Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy (FSM)
Published on: August 5, 2009
Real-time velocity measurement by the use of a speckle-pattern correlation system that incorporates a ferroelectric
Abstract:
We describe a technique for noncontact velocity measurement by using double-exposure speckle-pattern techniques with optical signal processing. The two speckle patterns are recorded on a ferroelectric liquid-crystal (FLC) spatial light modulator (SLM), which is a bistable optically addressed SLM, and the composite pattern is then analyzed by an optical system similar to a joint transform correlator, in which another FLC-SLM and a position-sensitive detector are used. We show that the performance of the system can be significantly improved by adjusting the time between exposures using a real-time feedback system that is based on the position of the correlation spot in the output plane.
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