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Updated: Jun 8, 2026
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Published on: August 9, 2024
Successful live birth from vitrified oocytes after 5 years of cryopreservation
Thomas J Kim1, Seung Wook Hong
1Reproductive Medicine Associates of New Jersey, Morristown, NJ, 07960, USA.
Objective:
To report a birth of a healthy boy after long-term cryopreservation of oocytes by vitrification.
Design:
Clinical application.
Setting:
IVF Center.
Patient:
A 17 year-old female with secondary pulmonary hypertension caused by transposition of great vessels visited our center in 2002, and she wished oocytes cryopreservation to avoid possible sterility after the following category X medication treatment.
Intervention(S):
Vitrified oocytes on Electron Microscope (EM) grids were warmed after 5 years of storage. Surviving MII oocytes were microinjected for fertilization and two embryos were transferred into a gestational carrier day 5 after microinjection.
Main Outcome Measure(S):
Survival, fertilization, cleavage, clinical pregnancy and delivery.
Result(S):
Eleven out of fourteen oocytes (78.6%) survived warming. Eight Metaphase II (MII) oocytes and 3 in vitro matured oocytes were microinjected; all 11 oocytes (100%) fertilized and 2 embryos were transferred on day 5. A healthy baby boy weighing 3,600 g was delivered at 38 weeks of gestation. Live-birth rates per warmed oocyte and per injected oocyte were 7.1% and 9.1% respectively.
Conclusion(S):
Cryopreservation after vitrification with EM grids maintained the developmental competence of oocytes after long-term storage and resulted in a successful live birth.
