Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Recombinant DNA

Recombinant DNA

Recombinant DNA

Recombinant DNA

Conservative Site-specific Recombination and Phase Variation

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Dissection and Engineering of Modular Polyketide Synthase Extender Unit Specificity Motifs.

Chembiochem : a European journal of chemical biology·2026

Same author

Deep learning-based automatic identification model of main pancreatic duct in intraoperative ultrasonography.

Surgical endoscopy·2026

Same author

Directed evolution of a cytochrome P450 monooxygenase for improved perillyl alcohol biosynthesis <i>via</i> a tailored genetically encoded biosensor.

RSC advances·2026

Same author

CogniSNN: Enabling neuron-expandability, pathway-reusability, and dynamic-configurability in spiking neural networks.

Neural networks : the official journal of the International Neural Network Society·2026

Same author

Engineering an Acyl-CoA Ligase With Enhanced Activity Toward Synthetic CoA Alternatives.

Chembiochem : a European journal of chemical biology·2026

Same author

Deciphering Early Oxidative Steps in Spirotetronate Biosynthesis Reveals a Two-Enzyme Cascade for Macrocyclic Lactone Formation.

Organic letters·2026

Search research articles

Related Experiment Video

Updated: Jun 8, 2026

The Production of C. elegans Transgenes via Recombineering with the galK Selectable Marker

The Production of C. elegans Transgenes via Recombineering with the galK Selectable Marker

Published on: January 11, 2011

Recombinant E. coli prototype strains for in vivo glycorandomization.

Gavin J Williams, Jie Yang, Changsheng Zhang

ACS Chemical Biology

|October 5, 2010

Summary

This study introduces in vivo glycorandomization using engineered E. coli for efficient small molecule glycoside production. This fermentation-based approach overcomes the scalability and cost limitations of in vitro methods.

More Related Videos

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays

Published on: November 12, 2012

The Multifaceted Benefits of Protein Co-expression in Escherichia coli

The Multifaceted Benefits of Protein Co-expression in Escherichia coli

Published on: February 5, 2015

Related Experiment Videos

Last Updated: Jun 8, 2026

The Production of C. elegans Transgenes via Recombineering with the galK Selectable Marker

The Production of C. elegans Transgenes via Recombineering with the galK Selectable Marker

Published on: January 11, 2011

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays

Published on: November 12, 2012

The Multifaceted Benefits of Protein Co-expression in Escherichia coli

The Multifaceted Benefits of Protein Co-expression in Escherichia coli

Published on: February 5, 2015

Area of Science:

Synthetic biology
Metabolic engineering
Biotechnology

Background:

In vitro glycorandomization is challenging to scale and expensive due to nucleotide and cofactor requirements.
Altering glycosylation patterns is crucial for natural products and small molecule therapeutics.

Discussion:

Engineered E. coli strains facilitate the production of diverse small molecule glycosides.
The strategy utilizes engineered enzyme promiscuity (anomeric kinase, sugar-1-phosphate nucleotidyltransferase, glycosyltransferase) and E. coli's uptake capabilities.
This represents the first demonstration of in vivo glycorandomization.

Key Insights:

Recombinant E. coli enables facile and scalable production of small molecule glycosides.
Engineered enzyme promiscuity is key to the in vivo glycorandomization process.
The method allows for combinatorial diversification of glycosylated compounds through fermentation.

Outlook:

Potential for high-throughput screening of novel glycosylated compounds.
Application in drug discovery and development pipelines.
Further optimization of E. coli strains for broader substrate scope and efficiency.