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Related Concept Videos

Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic cells are...
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...
EPS and iPS Cells in Disease Research01:21

EPS and iPS Cells in Disease Research

Embryonic and induced pluripotent stem cells are excellent models for disease research because of their ability to self-renew and differentiate into most cell types. Somatic cells from a patient are isolated and reprogrammed into induced pluripotent stem cells or iPSCs. These iPSCs are later differentiated into the desired cell type, which mirrors the diseased cell of the patient. In this way, disease models have been created for investigating diseases such as Down syndrome, type I diabetes,...
iPS Cell Differentiation01:22

iPS Cell Differentiation

The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012 for this...

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Related Experiment Video

Updated: Jun 8, 2026

Generation of Induced Pluripotent Stem Cells from Frozen Buffy Coats using Non-integrating Episomal Plasmids
10:52

Generation of Induced Pluripotent Stem Cells from Frozen Buffy Coats using Non-integrating Episomal Plasmids

Published on: June 5, 2015

Induced pluripotent stem (iPS) cell research overview.

Shih-Ping Liu1, Ru-Huei Fu, Yu-Chuen Huang

  • 1Center for Neuropsychiatry, China Medical University and Hospital, Taichung, Taiwan.

Cell Transplantation
|October 5, 2010
PubMed
Summary
This summary is machine-generated.

Induced pluripotent (iPS) cells offer a promising alternative to embryonic stem (ES) cells, overcoming immune rejection and ethical concerns. This review explores iPS cell generation methods and their clinical therapeutic potential.

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The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System
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Cryopreserving and Recovering of Human iPS Cells using Complete KnockOut Serum Replacement Feeder-Free Medium
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Last Updated: Jun 8, 2026

Generation of Induced Pluripotent Stem Cells from Frozen Buffy Coats using Non-integrating Episomal Plasmids
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Published on: June 5, 2015

The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System
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Cryopreserving and Recovering of Human iPS Cells using Complete KnockOut Serum Replacement Feeder-Free Medium
06:47

Cryopreserving and Recovering of Human iPS Cells using Complete KnockOut Serum Replacement Feeder-Free Medium

Published on: July 15, 2010

Area of Science:

  • Biotechnology
  • Stem Cell Biology
  • Regenerative Medicine

Background:

  • Embryonic stem (ES) cells possess self-renewal and differentiation capabilities for clinical applications.
  • ES cells face challenges including immune rejection and ethical considerations.
  • Induced pluripotent (iPS) cells were developed in 2006 from somatic cells using four key transcription factors.

Purpose of the Study:

  • To review the generation methods for iPS cells.
  • To compare iPS cells with ES cells.
  • To discuss the advantages and limitations of iPS cell technology for clinical applications.

Main Methods:

  • Generation of iPS cells from somatic cells (e.g., fibroblasts) using transcription factors (OCT4, SOX2, c-MYC, KLF4).
  • Comparison of iPS cell characteristics (morphology, proliferation, gene expression, etc.) with ES cells.
  • Evaluation of various iPS cell production strategies: retroviruses, lentiviruses, adenoviruses, plasmid transfections, transposons, and recombinant proteins.

Main Results:

  • iPS cells exhibit similar characteristics to ES cells, including pluripotency markers and epigenetic status.
  • iPS cells overcome ES cell limitations due to their derivation from autologous somatic cells.
  • Multiple methods exist for iPS cell generation, each with distinct advantages and disadvantages.

Conclusions:

  • iPS cells represent a significant advancement in cell therapy, addressing key limitations of ES cells.
  • The choice of iPS cell generation method impacts efficiency and safety.
  • Further research is needed to address clinical trial challenges, such as tumor formation and generation efficiency.