Related Experiment Video
Updated: Jun 8, 2026

Simultaneous pH Measurement in Endocytic and Cytosolic Compartments in Living Cells using Confocal Microscopy
Published on: April 28, 2014
An amphiphilic fluorescent probe for the visualization of histamine in living cells
Daisuke Seto1, Nobuaki Soh, Koji Nakano
1Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744, Moto-oka, Nishi-ku, Fukuoka 819-0395, Japan.
Abstract:
A novel fluorescent probe composed of two moieties, Nile Red and an iminodiacetic acid-Ni(2+) complex, for the detection of histamine in living cells is described. The probe was successfully applied to visualizing histamine in RAW264 cells, representing the first demonstration of the imaging of histamine itself in living cells.
Related Concept Videos
Labeling DNA Probes
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
FISH - Fluorescent In-situ Hybridization
Immunofluorescence Microscopy
The...

