Imaging fibroblast cells using atomic force microscopy (AFM)

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Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy
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Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy

Published on: July 10, 2019

Marija Plodinec, Marko Loparic, Ueli Aebi

    Cold Spring Harbor Protocols
    |October 5, 2010
    PubMed

    Abstract:

    Atomic force microscopy (AFM) can be used to visualize the three major cytoskeletal components that contribute to the mechanical properties of the cell. These are actin microfilaments, intermediate filaments, and microtubules. In this protocol, rat embryonic fibroblasts expressing actin tagged with green fluorescent protein (GFP) are used to demonstrate this procedure.

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    Atomic Force Microscopy01:08

    Atomic Force Microscopy

    Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
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    Studying the Cytoskeleton01:17

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    The cytoskeletal architecture can be studied using different microscopic and biochemical techniques. Electron microscopy was instrumental in discovering the cytoskeletal architecture around the 1960s, which allowed obtaining structural information at a high-resolution level. However, the sample preparation procedure often limits this ability in biological samples. Several protocols have been developed over the years to optimize sample preparation. In one of the protocols known as rotary...

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