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Related Concept Videos

Atomic Force Microscopy01:08

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
Studying the Cytoskeleton01:17

Studying the Cytoskeleton

The cytoskeletal architecture can be studied using different microscopic and biochemical techniques. Electron microscopy was instrumental in discovering the cytoskeletal architecture around the 1960s, which allowed obtaining structural information at a high-resolution level. However, the sample preparation procedure often limits this ability in biological samples. Several protocols have been developed over the years to optimize sample preparation. In one of the protocols known as rotary...

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Related Experiment Video

Updated: Jun 8, 2026

Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy
10:06

Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy

Published on: July 10, 2019

Imaging fibroblast cells using atomic force microscopy (AFM).

Marija Plodinec, Marko Loparic, Ueli Aebi

    Cold Spring Harbor Protocols
    |October 5, 2010
    PubMed
    Summary
    This summary is machine-generated.

    Atomic force microscopy visualizes key cell structures like actin, intermediate filaments, and microtubules. This protocol uses fluorescently tagged actin in rat cells to demonstrate the technique for studying cell mechanics.

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    Atomic Force Microscopy Imaging and Force Spectroscopy of Supported Lipid Bilayers

    Published on: July 22, 2015

    Related Experiment Videos

    Last Updated: Jun 8, 2026

    Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy
    10:06

    Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy

    Published on: July 10, 2019

    Measuring the Mechanical Properties of Living Cells Using Atomic Force Microscopy
    08:41

    Measuring the Mechanical Properties of Living Cells Using Atomic Force Microscopy

    Published on: June 27, 2013

    Atomic Force Microscopy Imaging and Force Spectroscopy of Supported Lipid Bilayers
    10:15

    Atomic Force Microscopy Imaging and Force Spectroscopy of Supported Lipid Bilayers

    Published on: July 22, 2015

    Area of Science:

    • Cell Biology
    • Biophysics
    • Microscopy

    Background:

    • The cell's mechanical properties are determined by its cytoskeleton.
    • Major cytoskeletal components include actin microfilaments, intermediate filaments, and microtubules.

    Purpose of the Study:

    • To demonstrate a protocol for visualizing cytoskeletal components using Atomic Force Microscopy (AFM).
    • To highlight the role of AFM in understanding cell mechanical properties.

    Main Methods:

    • Utilizing Atomic Force Microscopy (AFM) for high-resolution imaging.
    • Employing rat embryonic fibroblasts engineered to express green fluorescent protein (GFP)-tagged actin.
    • Visualizing actin microfilaments within the cellular structure.

    Main Results:

    • AFM successfully visualized the actin microfilaments in rat embryonic fibroblasts.
    • The protocol provides a method for observing a key cytoskeletal component.

    Conclusions:

    • Atomic force microscopy is a viable technique for visualizing cellular mechanical components.
    • This method aids in the study of cell structure and mechanics.