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Assay of hydroxyfarrerol in biological fluids
A Marzo1, E Arrigoni Martelli, G Bruno
1Drug Metabolism and Pharmacokinetic Department, Sigma-Tau SpA, Rome, Italy.
Journal of Chromatography
|December 28, 1990
Abstract:
A high-performance liquid chromatographic method for the determination of hydroxyfarrerol (IdB 1031) in biological samples was developed. IdB 1031 was first extracted by liquid-solid partition and the extracts were evaporated and analysed on a reversed-phase column under isocratic conditions, using either an electrochemical or a UV detector. The detection limit was ca. 5 ng/ml. Preliminary pharmacokinetic data showed that rats treated orally with 500 mg/kg had an average peak plasma concentration (Cmax) of 497 ng/ml after 2 h.