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Avidin-peroxidase: synthesis and HPLC isolation of highly sensitive oligomers
G V Gololobov1, A M Shuster, I K Zalite
1Engelhardt Institute of Molecular Biology, USSR Academy of Sciences, Moscow.
Journal of Biochemical and Biophysical Methods
|November 1, 1990
Abstract:
Optimal conditions for the preparation of avidin-peroxidase conjugates by the periodate method were studied. A method based on hydrophobic interaction chromatography was developed for the isolation of active oligomers. The probable structure of oligomers with highest sensitivity is discussed.