Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Antihypertensive Drugs: Angiotensin-Converting Enzyme Inhibitors01:30

Antihypertensive Drugs: Angiotensin-Converting Enzyme Inhibitors

871
Angiotensin-converting enzyme (ACE), a vital component of the renin-angiotensin-aldosterone system, is abundant in lung endothelial cells. ACE converts the inactive decapeptide, angiotensin I, into the active octapeptide, angiotensin II. This potent vasoconstrictor narrows blood vessels, increasing resistance to blood flow and elevating blood pressure. Angiotensin II also stimulates aldosterone production, encouraging kidney cells to reabsorb more sodium and water from urine, thereby increasing...
871
Overview of Cell-Matrix Interactions01:24

Overview of Cell-Matrix Interactions

7.5K
The extracellular matrix or ECM holds cells together to form a tissue and allows the cells within the tissue to communicate. ECM comprises proteins such as fibronectin, collagen, laminin, etc. The most abundant protein in this space is collagen. Collagen fibers are interwoven with carbohydrate-containing protein molecules called proteoglycans. ECM allows cell migration and provides a structural scaffold at cell adhesion that anchors the cell when the extracellular matrix proteins interact with...
7.5K
Heart Failure Drugs: Inhibitors of Renin-Angiotensin System01:26

Heart Failure Drugs: Inhibitors of Renin-Angiotensin System

505
The activation of the sympathetic nervous system and the renin-angiotensin-aldosterone system (RAAS) contributes to cardiac remodeling, and inhibiting the RAAS is a pharmacological target in heart failure management. As a result, neurohumoral modulation is a crucial treatment principle for managing heart failure. This approach involves using medications like ACE inhibitors (ACEIs), angiotensin receptor blockers (ARBs), β-blockers, mineralocorticoid receptor antagonists (MRAs), and neutral...
505
Antihypertensive Drugs: Direct Renin Inhibitors01:25

Antihypertensive Drugs: Direct Renin Inhibitors

817
The renin-angiotensin-aldosterone system (RAAS) is an intricate physiological pathway involving numerous enzymes and hormones, including renin, angiotensin-converting enzyme (ACE), angiotensin I and II, and aldosterone. Imbalances within this system increase the production of angiotensin II and aldosterone. Increased angiotensin II levels promote vasoconstriction and blood pressure elevation. Concurrently, higher aldosterone levels stimulate sodium and water reabsorption in the kidneys,...
817
Antihypertensive Drugs: Angiotensin II Receptor Blockers01:30

Antihypertensive Drugs: Angiotensin II Receptor Blockers

911
In the renin-angiotensin-aldosterone system, a hormone called angiotensin II plays a crucial role. It binds to the AT1 receptors in vascular smooth muscles coupled with Gq proteins. The activation of these receptors activates an enzyme called phospholipase C, which releases two molecules: inositol trisphosphate and diacylglycerol. These molecules cause a chain reaction that leads to the phosphorylation of myosin light chains and promotes interaction between actin and myosin, leading to smooth...
911
Role of Matrix Metalloproteases in Degradation of ECM01:23

Role of Matrix Metalloproteases in Degradation of ECM

2.5K
Matrix metalloproteases (MMPs) are enzymes involved in the hydrolysis of proteins and glycoproteins of the extracellular matrix. MMPs are essential for the migration and proliferation of cells through the dense matrix network, throughout embryonic development, and throughout morphogenesis. The first MMP activity discovered was a collagenase in a tadpole's tail undergoing metamorphosis. The active collagen deposition and modifications lead to the morphogenesis of tadpoles into the adult...
2.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pathomorphological Features of Lung Damage Caused by Infection with Various Variants of SARS-CoV-2 in Humanized Model Animals.

Doklady. Biochemistry and biophysics·2026
Same author

Isolation, Purification, and Structural-Functional Analysis of Recombinant Human Transferrin Produced by the Methylotrophic Yeast Strain Pichia pastoris.

Bulletin of experimental biology and medicine·2026
Same author

The Impact of the Intracellular Domains of Chimeric Antigenic Receptors on the Properties of CAR T-cells.

Acta naturae·2025
Same author

Heterologous Production of Antimicrobial Peptides in Yeast Allows for Massive Assessment of the Activity of DNA-Encoded Antimicrobials In Situ.

Acta naturae·2025
Same author

Detection and Quantification of Polymorphic MICA and MICB Molecules in Immunoassays: Initial Insights.

HLA·2025
Same author

Cell Model for Testing Pharmaceuticals Targeting Human PD-L1.

Sovremennye tekhnologii v meditsine·2025
Same journal

Reaction of the Blood System of ICR Mice with Traumatic Brain Injury.

Bulletin of experimental biology and medicine·2026
Same journal

Antidepressant Activity of 11-Dialkylaminoethyl-2,3,4,5-tetrahydrodiazepino[1,2-a]benzimidazole Derivative DAB-21 in a Model of Chronic Unpredictable Mild Stress.

Bulletin of experimental biology and medicine·2026
Same journal

Colon Adenocarcinoma Cell-Derived Galectins-1,3 Modulate Differentiation of CD4<sup>+</sup> T Lymphocytes In Vitro.

Bulletin of experimental biology and medicine·2026
Same journal

Circulating DNA-Markers of High Risk for Lung Cancer in Patients with Chronic Obstructive Pulmonary Disease.

Bulletin of experimental biology and medicine·2026
Same journal

Study of Anxiolytic Properties of Diterpene Alkaloid 12-Epinapelline N-Oxide.

Bulletin of experimental biology and medicine·2026
Same journal

Ethylmethylhydroxypyridine Succinate Limits Inflammatory-Degenerative Changes in the Hypothalamus and Corrects Disturbances in the Circadian Rhythm of Corticosterone Secretion in Aged Rats.

Bulletin of experimental biology and medicine·2026
See all related articles

Related Experiment Video

Updated: Sep 9, 2025

Receptor Autoradiography Protocol for the Localized Visualization of Angiotensin II Receptors
12:03

Receptor Autoradiography Protocol for the Localized Visualization of Angiotensin II Receptors

Published on: June 7, 2016

18.0K

Specific Matrix on the Basis of Angiotensin-Converting Enzyme 2: Development and Functional Analysis.

N N Kostin1, T V Bobik2, N Yu Rushkevich1

  • 1Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.

Bulletin of Experimental Biology and Medicine
|August 28, 2025
PubMed
Summary
This summary is machine-generated.

Researchers developed an affinity matrix using human angiotensin-converting enzyme 2 (ACE2) to capture SARS-CoV-2. This innovation shows promise for reducing viral load in severe COVID-19 patients through extracorporeal hemoperfusion.

Keywords:
COVID-19; extracorporeal hemoperfusion; ACE2

More Related Videos

Subcutaneous Angiotensin II Infusion using Osmotic Pumps Induces Aortic Aneurysms in Mice
07:21

Subcutaneous Angiotensin II Infusion using Osmotic Pumps Induces Aortic Aneurysms in Mice

Published on: September 28, 2015

37.9K
Nitropeptide Profiling and Identification Illustrated by Angiotensin II
07:31

Nitropeptide Profiling and Identification Illustrated by Angiotensin II

Published on: June 16, 2019

5.8K

Related Experiment Videos

Last Updated: Sep 9, 2025

Receptor Autoradiography Protocol for the Localized Visualization of Angiotensin II Receptors
12:03

Receptor Autoradiography Protocol for the Localized Visualization of Angiotensin II Receptors

Published on: June 7, 2016

18.0K
Subcutaneous Angiotensin II Infusion using Osmotic Pumps Induces Aortic Aneurysms in Mice
07:21

Subcutaneous Angiotensin II Infusion using Osmotic Pumps Induces Aortic Aneurysms in Mice

Published on: September 28, 2015

37.9K
Nitropeptide Profiling and Identification Illustrated by Angiotensin II
07:31

Nitropeptide Profiling and Identification Illustrated by Angiotensin II

Published on: June 16, 2019

5.8K

Area of Science:

  • Biotechnology
  • Virology
  • Materials Science

Background:

  • Severe COVID-19 is characterized by high viral loads.
  • Current treatments for severe COVID-19 have limitations.
  • Extracorporeal hemoperfusion is a potential method for blood purification.

Purpose of the Study:

  • To develop and characterize a novel affinity matrix for SARS-CoV-2 capture.
  • To evaluate the matrix's binding efficiency for viral particles.
  • To explore the potential of this matrix for therapeutic applications in COVID-19.

Main Methods:

  • Immobilization of recombinant human angiotensin-converting enzyme 2 (ACE2) onto a porous polymeric substrate.
  • Characterization of the developed affinity matrix.
  • Testing the matrix's binding capacity using viral particles pseudotyped with the SARS-CoV-2 spike protein.

Main Results:

  • A functional affinity matrix was successfully developed.
  • The matrix demonstrated efficient binding of SARS-CoV-2 pseudotyped viral particles.
  • The immobilized ACE2 maintained its binding properties on the substrate.

Conclusions:

  • The developed ACE2-based affinity matrix is a promising tool for capturing SARS-CoV-2.
  • This technology holds potential for extracorporeal hemoperfusion to reduce viral load in severe COVID-19.
  • Further research is warranted to translate this finding into clinical practice.