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DNA Vaccine Encoding the prM-E Polyprotein of the Tick-Borne Encephalitis Virus
E V Tigeeva1, V A Yakovlev2, M B Borgoyakova2
1State Research Center of Virology and Biotechnology "VECTOR", Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Koltsovo, Novosibirsk region, Russia. lena.tigeeva@gmail.com.
None:
We evaluated immunogenicity of the experimental DNA-vaccine construct pVAX-prM-E encoding the polyprotein prM-E of the tick-borne encephalitis virus (strain Sofjin). It was shown that pVAX-prM-E administered to mice using a needle-free jet injector induced the production of high levels of virus-neutralizing antibodies. The level of humoral response to pVAX-prM-E was comparable to that induced by administration of the commercial Tick-E-Vac vaccine. ELISpot analysis of the T-cell response showed that the obtained construct induces high levels of IFNγ in response to stimulation with a pool of specific E protein peptides. These findings indicate the high protective potential of this experimental DNA vaccine.
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