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FIA-spectrophotometric assay of N-acetylcysteine by o-phthalaldehyde derivatization
M J Medina Hernández1, M C García Alvarez-Coque, E Bonet Domingo
1Departamento de Química Analítica, Facultad de Química, Universidad de Valencia, Spain.
Die Pharmazie
|October 1, 1990
Abstract:
A flow injection-spectrophotometric procedure is proposed for the determination of N-acetylcysteine (NAC). The procedure is based on the rapid reaction of the thiol group with o-phthalaldehyde and isoleucine at pH 9.5 to give an 1-alkylthio-2-alkyl-substituted isoindole which shows maximum absorbance at 335 nm. The linear dynamic range is 16-160 micrograms/ml, with a 0.6% relative standard deviation for 100 micrograms/ml. The sampling rate is 126 samples/h. The method is applied successfully to the evaluation of NAC in commercial formulations.