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Updated: Aug 12, 2026

Isolation of Primary Murine Brain Microvascular Endothelial Cells
Published on: November 14, 2014
The role of endothelial second messenger's-generating system in the pathogenesis of brain oedema
1Laboratory of Molecular Neurobiology, Biological Research Center, Szeged, Hungary.
Abstract:
The role of second messengers in the regulation of protein phosphorylation was studied in microvessels isolated from rat cerebral cortex. Calcium-calmodulin (CAM)-, Ca2+/phospholipid (PK C)-, cyclic GMP (cGMP)-, and cyclic AMP (cAMP)-dependent protein kinases were detected. Autophosphorylation of both the alpha- and beta-subunits of CAM-dependent protein kinase and the proteolytic fragment of the PK C enzyme was also detected. In other experiments, the effect of the protein kinase C enzyme inhibitor H-7 was examined on the brain oedema formation evoked by bilateral occlusion of the common carotid arteries in Sprague-Dawley rats of CFY strain.
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