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Secondary cataract material collected with a glass cannula. A SEM study
W L Jongebloed1, G van der Veen, F Dijk
1Department of Histology and Cellbiology, University of Groningen, The Netherlands.
Documenta Ophthalmologica. Advances in Ophthalmology
|October 1, 1990
Summary
Secondary cataract material analysis reveals erythrocytes and inflammatory cells in early samples. Later samples show regenerated capsule epithelium and lens fiber fragments, indicating tissue regeneration post-surgery.
Area of Science:
- Ophthalmology
- Biomaterials Science
- Cell Biology
Background:
- Secondary cataracts can form after initial cataract surgery.
- Understanding the composition of secondary cataract material is crucial for managing post-operative complications.
Purpose of the Study:
- To characterize the composition of secondary cataract material collected at different time points after surgery.
- To investigate the cellular and tissue components present in secondary cataracts.
Main Methods:
- Secondary cataract material was collected from three patients at 18, 24, and 30 months post-operation.
- Scanning Electron Microscopy (SEM) was used for examination.
- Sample preparation involved filtration, fixation, drying, and centrifuging depending on material consistency.
Main Results:
- Material collected 18 months post-operation primarily contained erythrocytes, inflammatory cells, and degenerated lens fiber material.
- Material collected >20 months post-operation predominantly consisted of regenerated capsule epithelium fragments and solid lens fiber pieces with attached capsule epithelium.
Conclusions:
- Secondary cataracts can contain regenerated capsule epithelium and lens fiber fragments.
- The composition of secondary cataract material evolves over time after surgery.