Ultra-rapid high-performance liquid chromatographic screening for phenothiazines in human samples

Area of Science:

• Analytical Chemistry
• Pharmaceutical Analysis

Background:

• Phenothiazines are a class of widely prescribed antipsychotic medications.
• Accurate quantification of phenothiazines is crucial for therapeutic drug monitoring and pharmacokinetic studies.
• Existing analytical methods may lack efficiency or simultaneous detection capabilities for multiple phenothiazines.

Purpose of the Study:

• To develop and validate a high-performance liquid chromatographic (HPLC) method.
• To achieve simultaneous identification and quantification of six common phenothiazines.
• To establish an efficient extraction and analysis protocol for phenothiazine determination.

Main Methods:

• High-performance liquid chromatography (HPLC) with UV detection at 254 nm.
• Utilized a Spherisorb CN column and a mobile phase consisting of acetonitrile and acetate buffer (95:5).
• Employed a single-step alkaline extraction using heptane-isoamyl alcohol (98.5:1.5) with prochlorperazine as an internal standard.

Main Results:

• The method successfully achieved simultaneous identification and quantification of six phenothiazines.
• A single-step extraction procedure simplified sample preparation.
• The HPLC conditions provided good separation and detection sensitivity.

Conclusions:

• The developed HPLC method is effective for the simultaneous analysis of common phenothiazines.
• This method offers a reliable and efficient approach for phenothiazine quantification in analytical and clinical settings.
• The technique can aid in therapeutic drug monitoring and pharmaceutical quality control.