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Updated: Jun 8, 2026

Measuring the Osmotic Water Permeability Coefficient (Pf) of Spherical Cells: Isolated Plant Protoplasts as an Example
Published on: October 8, 2014
A universal self-adaptive time-varying function for extracellular concentration during osmotic shift for
1Department of Modern Mechanics, University of Science and Technology of China, Hefei 230027, PR China. zhaog@ustc.edu.cn
Abstract:
A universal self-adaptive time-varying function of extracellular concentration history during osmotic shift for measuring cell membrane permeability was presented in this study. The feasibility and accuracy of the assumed function were verified based on the experimental data obtained from the microperfusion chamber method. It was found that the assumed function could always give out the very satisfactory coefficient of determination, and there were no significant differences between the hydraulic conductivity values fitted using the laser interferometer measured extracellular concentration profile and the predicted one by the assumed piecewise function (student's t test, p > 0.05). Due to the adaptive feature of the assumed function for the concentration of extracellular solution, the function was suggest to be used for all the similar studies for measurement of cell membrane permeability by osmotic shift.
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