Solid lipid nanoparticles (SLNs) derived from para-acyl-calix[9]-arene: preparation and stability

Area of Science:

• Materials Science
• Nanotechnology
• Pharmaceutical Sciences

Background:

• Solid lipid nanoparticles (SLNs) are crucial drug delivery systems.
• Para-acyl-calix[9]arene derivatives offer a novel platform for SLN formulation.

Purpose of the Study:

• To investigate preparation parameters influencing para-acyl-calix[9]arene-based SLN characteristics.
• To assess the stability and protein interaction of these novel SLNs.

Main Methods:

• Dynamic light scattering and electron microscopy for particle size analysis.
• Systematic variation of preparation parameters (solvent, concentration, co-surfactant, ionic strength).
• Stability testing under various conditions (temperature, freeze-thaw, protein presence).

Main Results:

• Particle size ranged from 85-215 nm, influenced by acyl chain length, solvent, amphiphile concentration, and co-surfactant.
• SLN stability was salt-dependent and improved by mono- or disaccharides during freeze-thaw cycles.
• Temporal stability in water exceeded 6 months; no aggregation with human serum albumin observed.
• Successful encapsulation demonstrated using acridine as a fluorescent probe.

Conclusions:

• Preparation parameters significantly impact para-acyl-calix[9]arene SLN size and stability.
• These SLNs exhibit promising long-term stability and good protein compatibility.
• The developed SLNs are suitable for cryopreservation and show potential for encapsulation applications.