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Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

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Related Experiment Video

Updated: Jun 8, 2026

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
08:40

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

Desperately seeking microRNA targets.

Marshall Thomas1, Judy Lieberman, Ashish Lal

  • 1Immune Disease Institute and Program in Cellular and Molecular Medicine, Children's Hospital Boston, Harvard Medical School, Boston, Massachusetts, USA.

Nature Structural & Molecular Biology
|October 7, 2010
PubMed
Summary
This summary is machine-generated.

MicroRNAs (miRNAs) regulate gene expression by targeting messenger RNAs. Identifying these miRNA-regulated genes is crucial for understanding cellular responses and is achieved through combined computational and experimental methods.

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Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells
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Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells

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Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs
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Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

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Related Experiment Videos

Last Updated: Jun 8, 2026

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
08:40

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells
07:19

Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells

Published on: September 28, 2011

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs
11:00

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

Published on: June 12, 2018

Area of Science:

  • Molecular Biology
  • Genetics
  • Bioinformatics

Background:

  • MicroRNAs (miRNAs) are key regulators of gene expression, influencing processes like development and environmental responses.
  • Each miRNA can target hundreds of genes, making their functional analysis complex.
  • Identifying miRNA targets is challenging due to partial complementarity and subtle expression changes.

Purpose of the Study:

  • To review computational and experimental approaches for identifying genes regulated by miRNAs.
  • To highlight methods for understanding miRNA function through target gene identification.

Main Methods:

  • Review of computational algorithms for miRNA target prediction.
  • Examination of experimental methods, including gene expression analysis and transcript isolation.
  • Bioinformatic analysis of pathways and protein-DNA interactomes.

Main Results:

  • Integrated approaches combining computational predictions with experimental validation are effective.
  • Analyzing expression changes upon altered miRNA levels aids target identification.
  • Bioinformatic analysis of candidate target lists reveals biologically significant genes.

Conclusions:

  • A combination of computational and experimental strategies is essential for accurate miRNA target identification.
  • Understanding miRNA-regulated genes is critical for deciphering cellular regulatory networks.