Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
Surface Active Agents01:27

Surface Active Agents

Surfactants, named for their behavior at interfaces, positively adsorb at the interfaces of two phases, reducing interfacial tension. Their versatility as emulsifiers, detergents, and foaming agents stems from this ability. Surfactants, often termed amphiphiles, share the property of amphipathy, with molecules having both hydrophilic and hydrophobic portions. The hydrophilic part is called the head, and the hydrophobic part, including an elongated alkyl substituent, forms the tail.Surfactants...
Analyte Adsorption and Distribution01:09

Analyte Adsorption and Distribution

In certain chromatographic separations, solutes transfer between the mobile phase and the stationary phase via sorption, which typically refers to the process of adsorption. For many chromatographic systems, the sorption process often depends on the polarity of the compounds—an expression of the overall dipole moment within the molecule. During the separation process, there is competition between the solute and solvent for adsorption to the stationary phase. Highly polar compounds and solvents...
Micelles01:30

Micelles

Micelle formation is an intricate process that hinges on the properties of amphiphilic or amphipathic molecules and the conditions of the system in which they are found. Amphiphilic molecules, which have both hydrophilic (water-attracting) and hydrophobic (water-repelling) parts, play a critical role in this process.In aqueous environments, these molecules arrange themselves such that their hydrophilic heads are turned towards the water phase, while their hydrophobic tails are oriented away...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

From Epithelial Sensing to Visceral Pain: Neuropod and Enterochromaffin Cells in Gut Neuroepithelial Circuits.

International journal of molecular sciences·2026
Same author

Chain length dependent stabilization of charged nanoplatelet dispersions by inorganic polyphosphates.

Journal of colloid and interface science·2026
Same author

Antibiotic treatment reveals the contributions of the gut microbiome to CLN2 disease in the central and enteric nervous system.

Scientific reports·2026
Same author

Data-driven classification of tissue water populations by massively multidimensional diffusion-relaxation correlation MRI.

Frontiers in neuroscience·2026
Same author

Awake Craniotomy Versus General Anesthesia for Resection of High-Grade Gliomas: A Systematic Review and Meta-Analysis.

Journal of clinical medicine·2026
Same author

Jasmonate signaling and prey nutrient availability trigger distinct biochemical responses in the Drosera capensis feeding cycle.

Plant physiology·2026
Same journal

Interfacial Behavior and Adsorption Mechanisms of Sorbitol Polyether Ester Emulsifiers in D-Phase Emulsion Systems: Applications for Spontaneous Emulsification.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Ti<sub>3</sub> Cluster-Doped 2D Goldene Surface for Electronic and Optical Sensing of Oxygen, Nitrogen, and Hydrocarbon-Based Respiratory Biomarkers: A DFT Study.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Condensation of GO<i>x</i> by Restoring Global Protein Fold on Diazo-Enriched SG Surfaces: Reinstallation of FADs Restores Biocatalytic Sustainability in Quantifying Blood Glucose.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Correction to "Protein Encapsulated DNA Metal Nanocluster as Fluorescent Nanoprobe for Detecting Insulin".

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Rational Design of V<sub>2</sub>O<sub>5</sub> Hierarchical Microspheres with Tunable Porosities and Primary Building Blocks for Enhanced Lithium Storage Performance.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Roles of Anion and Cation Doping in g-C<b><sub>3</sub></b>N<b><sub>4</sub></b> as Artificial SEI for Regulating Interfacial Zn<b><sup>2+</sup></b> Deposition in Aqueous Zinc Metal Anodes.

Langmuir : the ACS journal of surfaces and colloids·2026
See all related articles

Related Experiment Video

Updated: Jun 8, 2026

Phase Diagram Characterization Using Magnetic Beads as Liquid Carriers
12:37

Phase Diagram Characterization Using Magnetic Beads as Liquid Carriers

Published on: September 4, 2015

Polarization transfer solid-state NMR for studying surfactant phase behavior.

Agnieszka Nowacka1, Parveen Choudhary Mohr, Jens Norrman

  • 1Physical Chemistry, POB 124, Lund University, SE-221 00 Lund, Sweden. agnieszka.nowacka@fkem1.lu.se

Langmuir : the ACS Journal of Surfaces and Colloids
|October 8, 2010
PubMed
Summary
This summary is machine-generated.

This study introduces a (13)C solid-state NMR technique to characterize amphiphile phase behavior at low water content. The method distinguishes solid, liquid crystalline, and isotropic phases by analyzing molecular conformation and dynamics.

More Related Videos

Neutron Spin Echo Spectroscopy as a Unique Probe for Lipid Membrane Dynamics and Membrane-Protein Interactions
10:02

Neutron Spin Echo Spectroscopy as a Unique Probe for Lipid Membrane Dynamics and Membrane-Protein Interactions

Published on: May 27, 2021

Related Experiment Videos

Last Updated: Jun 8, 2026

Phase Diagram Characterization Using Magnetic Beads as Liquid Carriers
12:37

Phase Diagram Characterization Using Magnetic Beads as Liquid Carriers

Published on: September 4, 2015

Neutron Spin Echo Spectroscopy as a Unique Probe for Lipid Membrane Dynamics and Membrane-Protein Interactions
10:02

Neutron Spin Echo Spectroscopy as a Unique Probe for Lipid Membrane Dynamics and Membrane-Protein Interactions

Published on: May 27, 2021

Area of Science:

  • Solid-state NMR spectroscopy
  • Amphiphile phase behavior
  • Materials science

Background:

  • Amphiphiles like lipids and surfactants are crucial in technical and pharmaceutical applications.
  • Characterizing amphiphile phase behavior at low water content is challenging due to phase coexistence.
  • Understanding phase behavior is key for optimizing applications.

Purpose of the Study:

  • To develop and validate a (13)C solid-state NMR technique for investigating amphiphile phase behavior in the water-poor regime.
  • To differentiate between solid crystalline, solid amorphous, anisotropic liquid crystalline, and isotropic liquid crystalline phases.
  • To determine phase diagrams by combining information on molecular conformation and dynamics.

Main Methods:

  • Utilizing (13)C solid-state NMR with Carr-Purcell (CP) and Insensitive Nuclei Enhanced by Polarization Transfer (INEPT) pulse sequences.
  • Analyzing (13)C chemical shifts for molecular conformation and signal intensities for molecular dynamics (correlation time τ(c) and order parameter S).
  • Applying theoretical analysis to correlate NMR signal intensities with molecular motion parameters.

Main Results:

  • Demonstrated that CP and INEPT signal intensities provide distinct information on molecular dynamics, aiding phase identification.
  • Successfully mapped the temperature-composition phase diagram for the cetyltrimethylammonium succinate/water system, identifying multiple solid and liquid crystalline phases.
  • Showed that replacing succinate with DNA alters phase formation and increases molecular dynamics correlation times.

Conclusions:

  • The described (13)C solid-state NMR technique offers a facile method for determining amphiphile phase diagrams in the water-poor regime.
  • The technique effectively distinguishes between various solid and liquid crystalline phases based on molecular conformation and dynamics.
  • The study highlights the influence of counterions on amphiphile phase behavior and molecular dynamics.