[Intravital estimation of doxorubicin accumulation in various types of cell cultures]

An extractionless procedure was developed for intravital quantitative estimation of doxorubicin (DR) accumulation in cell cultures with using fluorescence measurements. DR accumulation in a monolayer culture was evaluated by a decrease in the fluorescence of the incubation medium or by the total inhibition of the fluorescence of the cells and medium in the suspension medium. It was shown that there was a definite range of the doses from 3.10(-8) to 2.10(-6) M at which the accumulation kinetics recorded subsequently in one bottle for any two concentrations of the above ranges did not depend on the antibiotic dose. In this connection, the procedure was approved for quantitative estimation of DR accumulation in the same cells before and after modifying effects.