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Simple Elimination of Background Fluorescence in Formalin-Fixed Human Brain Tissue for Immunofluorescence Microscopy
Published on: September 3, 2017
Structured illumination microscopy of autofluorescent aggregations in human tissue
Gerrit Best1, Roman Amberger1, David Baddeley2
1Applied Optics and Information Processing, Kirchhoff-Institute for Physics, University of Heidelberg, Heidelberg, Germany.
Abstract:
Sections from human eye tissue were analyzed with Structured Illumination Microscopy (SIM) using a specially designed microscope setup. In this microscope the structured illumination was generated with a Twyman-Green Interferometer. This SIM technique allowed us to acquire light-optical images of autofluorophore distributions in the tissue with previously unmatched optical resolution. In this work the unique setup of the microscope made possible the application of SIM with three different excitation wavelengths (488, 568 and 647 nm), thus enabling us to gather spectral information about the autofluorescence signal.
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