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Detection of Post-translational Modifications on Native Intact Nucleosomes by ELISA
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Identification of epigenetic DNA modifications with a protein nanopore

Emma V B Wallace1, David Stoddart, Andrew J Heron

  • 1Department of Chemistry, University of Oxford, Chemistry Research Laboratory, Mansfield Road, Oxford, UK OX1 3TA.

Chemical Communications (Cambridge, England)
|October 8, 2010
PubMed

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Chromatin Immunoprecipitation- ChIP02:36

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Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
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ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...