Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Voltammetric Techniques: Linear-Scan (E vs Time)01:12

Voltammetric Techniques: Linear-Scan (E vs Time)

Polarography is a classical voltammetric technique used to analyze electrochemical reactions. This method applies a linear potential sweep to a dropping mercury electrode (DME), and the resulting current is measured. A dropping mercury electrode is commonly used as the working electrode in polarography. It consists of a capillary tube filled with mercury, where the tiny droplet forms at the tip. This droplet continuously drops from the capillary, creating a new electrode surface for each...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Normal Values for Atrial Deformation Measured by Feature-Tracking Cardiac MRI: A Meta-Analysis.

Journal of magnetic resonance imaging : JMRI·2024
Same author

Two new clades recovered at high temperatures provide novel phylogenetic and genomic insights into <i>Candidatus</i> Accumulibacter.

ISME communications·2024
Same author

GLP-1 receptor agonists and myocardial metabolism in atrial fibrillation.

Journal of pharmaceutical analysis·2024
Same author

Study on uneven settlement and correction of steel frame structures based on numerical simulation method.

PloS one·2024
Same author

Relating the carbon sources to denitrifying community in full-scale wastewater treatment plants.

Chemosphere·2024
Same author

Structure of Polysaccharide from <i>Dendrobium nobile</i> Lindl. and Its Mode of Action on TLR4 to Exert Immunomodulatory Effects.

Foods (Basel, Switzerland)·2024

Related Experiment Video

Updated: Jun 8, 2026

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
14:53

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

Label-free voltammetric detection using individually addressable oligonucleotide microelectrode arrays.

Roya Kalantari1, Ryan Cantor, Hang Chen

  • 1School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia 30332, United States.

Analytical Chemistry
|October 9, 2010
PubMed
Summary

Label-free oligonucleotide probes on microelectrode arrays enable reagentless detection of microbial targets like E. coli and C. albicans. This voltammetric method offers a "yes-no" answer for specific binding, despite potential cross-reactivity challenges.

More Related Videos

Bacterial Detection &amp; Identification Using Electrochemical Sensors
09:30

Bacterial Detection & Identification Using Electrochemical Sensors

Published on: April 23, 2013

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules
13:15

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules

Published on: June 1, 2011

Related Experiment Videos

Last Updated: Jun 8, 2026

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
14:53

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

Bacterial Detection &amp; Identification Using Electrochemical Sensors
09:30

Bacterial Detection & Identification Using Electrochemical Sensors

Published on: April 23, 2013

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules
13:15

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules

Published on: June 1, 2011

Area of Science:

  • Biosensors
  • Electrochemistry
  • Nucleic Acid Detection

Background:

  • Label-free detection methods are crucial for simplifying diagnostic assays.
  • Oligonucleotide probes offer high specificity for target analyte identification.
  • Voltammetric transduction provides a sensitive electrochemical readout principle.

Purpose of the Study:

  • To evaluate the performance of label-free oligonucleotide probes for reagentless detection of dilute analytes.
  • To assess the utility of a voltammetric microelectrode array for specific nucleic acid detection.
  • To investigate probe-specific interactions and potential challenges in probe selection strategies.

Main Methods:

  • Fabrication of preproduction arrays with 16 individually addressable microelectrodes.
  • Immobilization of 2 -O-methylribonucleic acid and DNA backbone probes onto polypyrrole films.
  • Detection of microbial target RNAs (E. coli 23S rRNA, C. albicans 18S rRNA) using voltammetric transduction.
  • Analysis of probe specificity and cross-reactivity with non-target transcripts.

Main Results:

  • Detection of E. coli 23S rRNA at 65 fmol/mL and C. albicans 18S rRNA at 58 fmol/mL in 0.5 mL volumes.
  • Demonstrated probe-specific interactions with target microbial RNAs.
  • Identified that non-target transcripts can influence the voltammetric signal but do not exceed 70% of specific target signals.
  • Statistically validated the voltammetric microelectrode array for "yes-no" specific binding determination.

Conclusions:

  • The developed voltammetric microelectrode array with label-free oligonucleotide probes is effective for reagentless detection of specific microbial targets.
  • The system provides a reliable "yes-no" indication of complementary binding, crucial for diagnostic applications.
  • Challenges in oligonucleotide probe selection strategies require careful consideration to optimize specificity and minimize false positives.