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Anti-virulent Disruption of Pathogenic Biofilms using Engineered Quorum-quenching Lactonases
Published on: January 1, 2016
[Advances in lactamases from microbes--a review]
Jianjun Wang1, Guojun Zheng, Sheng Wu
1Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China. wangjj@im.ac.cn
Abstract:
gamma-Lactamase belongs to the amidase. The (+) gamma-lactamses can be applied in the kinetic resolution of racemic gamma-lactam, which can produce (-) gamma-lactam efficiently. The (-) gamma-lactam enantiomer is an important synthon for the synthesis of carbocyclic nucleosides. Up to now seven strains of microbes were reported that produced gamma-lactamses. The crystal structure of (-) lactamase from Aureoacterium sp. was resoluted, and the catalytic mechanism based on the structure data analysis was alike with the alpha/beta hydrolase family. However, no structure data and mechanism theory is available for (+) lactamase till now. The future works focus on protein engineering of the gamma-lactamase to improve the catalysis of the protein, elucidation of the catalytic mechanism of the gamma-lactamase and the functions of the gamma-lactamase in vivo.
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