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Updated: Jun 8, 2026

A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries
Published on: February 1, 2011
Selection and characteristics of a switchgrass-colonizing microbial community to produce extracellular cellulases and
HongYan Yang1, Hao Wu, XiaoFen Wang
1College of Life Sciences, Northeast Forestry University, Harbin, Heilongjiang, China.
Abstract:
A microbial community was selected for growth on dried and NaOH-treated switchgrass. During a 14-day liquid cultivation, a 70% loss in dry weight was observed during the first 4 days and after 14 days, the hemicellulose and cellulose in the system were degraded by 73.5% and 67.3%, respectively. The carboxymethyl cellulase (CMCase) and xylanase levels reached 0.21 and 3.75 IU, respectively. The optimal pH for CMCase and xylanase activities was 5 and 6, respectively. The optimal reaction temperature of CMCase and xylanase was 60°C. A library of bacterial and fungal ribosomal gene sequences obtained from the community showed the presence of Achromobacter xylosoxidans and Alcaligenes faecalis and of Fusarium sporotrichioides. To our knowledge, this was the first report on a microbial community selected in the presence of switchgrass to produce extracellular cellulases and xylanases.
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