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Updated: Jun 8, 2026
Dynamic Multiparameter Platelet Function Assessment Using a Capacitive Biosensor
Published on: May 2, 2025
Platelet function analysis with two different doses of aspirin
Alp Aydinalp1, Ilyas Atar, Cihan Altin
1Department of Cardiology, Medicine Faculty of Başkent University, Ankara, Turkey. aydinkalp@superonline.com
Insights
Higher aspirin doses (300 mg) improve platelet inhibition compared to lower doses (100 mg), particularly in women. This study highlights aspirin dose and female sex as key factors in achieving effective platelet inhibition.
Area of Science:
- Cardiology
- Pharmacology
- Hematology
Background:
- Aspirin is a widely used antiplatelet medication.
- Assessing the effectiveness of different aspirin dosages is crucial for optimizing patient outcomes.
- Platelet function testing provides objective measures of antiplatelet therapy efficacy.
Purpose of the Study:
- To compare platelet inhibition levels between patients receiving low-dose (100 mg) versus medium-dose (300 mg) aspirin.
- To identify factors associated with incomplete platelet inhibition despite aspirin therapy.
Main Methods:
- Prospective study of 159 cardiology outpatients on 100 mg or 300 mg daily aspirin.
- Platelet reactivity assessed using the Platelet Function Analyzer (PFA)-100 system.
- Incomplete platelet inhibition defined as normal collagen/epinephrine closure time (< 165 sec).
Main Results:
- Overall, 22% of patients exhibited incomplete platelet inhibition.
- Incomplete inhibition was significantly more prevalent in the 100 mg aspirin group (30.4%) compared to the 300 mg group (13.8%) (p=0.013).
- Female sex and lower aspirin dose were independent predictors of incomplete platelet inhibition.
Conclusions:
- Higher aspirin doses (300 mg) are associated with reduced incomplete platelet inhibition.
- Female patients may be more susceptible to incomplete platelet inhibition.
- Optimizing aspirin dosage may be particularly important for female patients to ensure adequate platelet inhibition.
Objectives:
We aimed to compare the level of platelet inhibition using the platelet function analyzer (PFA)-100 in patients receiving low and medium doses of aspirin.
Study Design:
On a prospective basis, 159 cardiology outpatients (83 men, 76 women; mean age 60.9 ± 9.9 years) taking 100 mg/day or 300 mg/day aspirin at least for the previous 15 days were included. Of these, 79 patients (50%) were on 100 mg and 80 patients (50.3%) were on 300 mg aspirin treatment. Blood samples were collected between 09:30 and 11:00 hours in the morning. Platelet reactivity was measured with the PFA-100 system. Incomplete platelet inhibition was defined as a normal collagen/epinephrine closure time (< 165 sec) despite aspirin treatment.
Results:
Baseline clinical and laboratory characteristics of the patient groups taking 100 mg or 300 mg aspirin were similar. The overall prevalence of incomplete platelet inhibition was 22% (35 patients). The prevalence of incomplete platelet inhibition was significantly higher in patients treated with 100 mg of aspirin (n = 24/79, 30.4%) compared with those treated with 300 mg of aspirin (n = 11/80, 13.8%) (p = 0.013). In univariate analysis, female sex (p = 0.002) and aspirin dose (p = 0.013) were significantly correlated with incomplete platelet inhibition. In multivariate analysis, female sex (OR: 0.99; 95% CI 0.9913-0.9994; p = 0.025) and aspirin dose (OR: 3.38; 95% CI 1.4774-7.7469; p = 0.003) were found as independent factors predictive of incomplete platelet inhibition.
Conclusion:
Our findings suggest that treatment with higher doses of aspirin can reduce incomplete platelet inhibition especially in female patients.
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