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Updated: Jun 8, 2026

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis
Published on: April 17, 2017
Efficient hepatitis C virus particle formation requires diacylglycerol acyltransferase-1
Eva Herker1, Charles Harris, Céline Hernandez
1Gladstone Institute of Virology and Immunology, University of California, San Francisco, USA.
Abstract:
Hepatitis C virus (HCV) infection is closely tied to the lipid metabolism of liver cells. Here we identify the triglyceride-synthesizing enzyme diacylglycerol acyltransferase-1 (DGAT1) as a key host factor for HCV infection. DGAT1 interacts with the viral nucleocapsid core and is required for the trafficking of core to lipid droplets. Inhibition of DGAT1 activity or RNAi-mediated knockdown of DGAT1 severely impairs infectious virion production, implicating DGAT1 as a new target for antiviral therapy.
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