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Updated: Jun 8, 2026

Mitochondrial Transformation in Baker's Yeast to Study Translation and Respiratory Complex Assembly
Published on: June 7, 2024
Chemical synthesis of the mouse mitochondrial genome
Daniel G Gibson1, Hamilton O Smith, Clyde A Hutchison
1The J. Craig Venter Institute, Rockville, Maryland, USA. dgibson@jcvi.org
Abstract:
We describe a one-step, isothermal assembly method for synthesizing DNA molecules from overlapping oligonucleotides. The method cycles between in vitro recombination and amplification until the desired length is reached. As a demonstration of its simplicity and robustness, we synthesized the entire 16.3-kilobase mouse mitochondrial genome from 600 overlapping 60-mers.
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