Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Types Of Column Chromatography01:29

Types Of Column Chromatography

The stability and compatibility of column material with samples are crucial for efficient purification in chromatographic techniques. Various operating parameters such as pH, temperature, or solvent affect the packing of the column material, thereby determining the purification efficiency. The choice of column material also plays an essential role in deciding the operating parameters and can be modified based on the proteins that need to be purified.
Gel Filtration Chromatography
When the...
Affinity Chromatography01:03

Affinity Chromatography

Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
Principles Of Column Chromatography01:13

Principles Of Column Chromatography

The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...
Size-Exclusion Chromatography01:08

Size-Exclusion Chromatography

In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
Silica particles offer advantages such as rigidity,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Modulating Membrane Surface Properties via Prewetting With Polysorbate 20 to Improve Sterile Filtration of Nanoemulsions.

Biotechnology journal·2026
Same author

Application of commercial ultrafiltration membranes for protein purification by high performance countercurrent membrane purification (HPCMP).

Biotechnology progress·2026
Same author

Hydrogenation Kinetics Study: Precise Control of C=C Bonds in Polyisoprene (PI)-Containing Block Copolymers via Diimide Hydrogenation.

ACS applied polymer materials·2026
Same author

Longitudinal Tracking of Astrocyte Reactivity During the Development of Chronic Orofacial Neuropathic Pain Using [<sup>18</sup>F]-SMBT-1 Positron-Emission Tomography.

Glia·2026
Same author

Viscosity of concentrated mRNA solutions: Implications for downstream processing.

Biotechnology progress·2026
Same author

Continuous Purification of mRNA Produced by In Vitro Transcription Using High Performance Countercurrent Membrane Purification.

Biotechnology and bioengineering·2026

Related Experiment Video

Updated: Jun 8, 2026

An Economical and Versatile High-Throughput Protein Purification System Using a Multi-Column Plate Adapter
10:08

An Economical and Versatile High-Throughput Protein Purification System Using a Multi-Column Plate Adapter

Published on: May 21, 2021

Countercurrent tangential chromatography for large-scale protein purification.

Oleg Shinkazh1, Dharmesh Kanani, Morgan Barth

  • 1Chromatan, Inc., Dedham, MA, USA.

Biotechnology and Bioengineering
|October 13, 2010
PubMed
Summary

Countercurrent tangential chromatography offers a solution to the downstream bottleneck in therapeutic protein purification. This method achieves high-purity protein separation with reduced buffer consumption, demonstrating its potential for large-scale bioprocessing.

More Related Videos

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification
10:21

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification

Published on: September 21, 2011

Automated Counterflow Centrifugal System for Small-Scale Cell Processing
04:49

Automated Counterflow Centrifugal System for Small-Scale Cell Processing

Published on: December 12, 2019

Related Experiment Videos

Last Updated: Jun 8, 2026

An Economical and Versatile High-Throughput Protein Purification System Using a Multi-Column Plate Adapter
10:08

An Economical and Versatile High-Throughput Protein Purification System Using a Multi-Column Plate Adapter

Published on: May 21, 2021

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification
10:21

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification

Published on: September 21, 2011

Automated Counterflow Centrifugal System for Small-Scale Cell Processing
04:49

Automated Counterflow Centrifugal System for Small-Scale Cell Processing

Published on: December 12, 2019

Area of Science:

  • Biotechnology
  • Biochemical Engineering
  • Protein Purification

Background:

  • Advances in cell culture increase demand for efficient downstream purification of recombinant therapeutic proteins.
  • The "downstream bottleneck" hinders the production of vital protein-based therapies for diseases like cancer and cardiovascular disorders.
  • Conventional chromatography methods face limitations in scalability and efficiency.

Purpose of the Study:

  • To demonstrate the feasibility of countercurrent tangential chromatography (CTC) for protein purification.
  • To evaluate CTC's effectiveness in separating a model protein mixture.
  • To assess the potential of CTC for large-scale therapeutic protein production.

Main Methods:

  • Utilized countercurrent tangential chromatography with a slurry of anion exchange resin flowing through static mixers and hollow fiber modules.
  • Employed batch uptake/desorption experiments and critical flux data for system design.
  • Separated a model protein mixture of bovine serum albumin and myoglobin.

Main Results:

  • Achieved >99% purity of the target protein with 94% recovery in a two-stage batch separation.
  • Demonstrated high-resolution separation capabilities of CTC.
  • Showcased reduced buffer requirements compared to conventional methods.

Conclusions:

  • Countercurrent tangential chromatography is a feasible and effective method for protein purification.
  • CTC addresses the downstream bottleneck in biopharmaceutical manufacturing.
  • This technique holds significant potential for the large-scale purification of therapeutic proteins.