Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Telomeres and Telomerase02:41

Telomeres and Telomerase

In eukaryotic DNA replication, a single-stranded DNA fragment remains at the end of a chromosome after the removal of the final primer. This section of DNA cannot be replicated in the same manner as the rest of the strand because there is no 3’ end to which the newly synthesized DNA can attach. This non-replicated fragment results in gradual loss of the chromosomal DNA during each cell duplication. Additionally, it can induce a DNA damage response by enzymes that recognize single-stranded DNA.
Replicative Cell Senescence02:15

Replicative Cell Senescence

Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds the telomeric...
Non-LTR Retrotransposons03:18

Non-LTR Retrotransposons

As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Transfection of IFNalpha in human glioblastoma cells and tumorigenicity in association with induction of PKR and OAS gene expression.

Journal of neurosurgery·1996
Same author

In vitro transcriptional analysis of TyrR-mediated activation of the mtr and tyrP+3 promoters of Escherichia coli.

Journal of bacteriology·1996
Same author

Case report: ranitidine-induced bradycardia in a patient with dextrocardia.

The American journal of the medical sciences·1996
Same author

Antioxidant-sensitive regulation of inflammatory-response genes in Kaposi's sarcoma cells.

Journal of acquired immune deficiency syndromes and human retrovirology : official publication of the International Retrovirology Association·1996
Same author

Cytokine expression in the presence or absence of late airway responses after antigen challenge of sensitized rats.

American journal of respiratory cell and molecular biology·1996
Same author

Injuries of the abdominal aorta and inferior vena cava in association with thoracolumbar fractures: a lethal combination.

The Journal of trauma·1996

Related Experiment Video

Updated: Jun 8, 2026

Semi-quantitative Detection of RNA-dependent RNA Polymerase Activity of Human Telomerase Reverse Transcriptase Protein
08:26

Semi-quantitative Detection of RNA-dependent RNA Polymerase Activity of Human Telomerase Reverse Transcriptase Protein

Published on: June 12, 2018

[Telomerase activity in human primary lung caner.].

J Yang1, X Li, Q Li

  • 1Respiratory Department , First Affliated Hospital of Hubei Medical University , Wuhan , Hubei 430060 , P. R. China.

Zhongguo Fei AI Za Zhi = Chinese Journal of Lung Cancer
|October 14, 2010
PubMed
Summary

Telomerase activity is significantly higher in primary lung cancer tissues than in benign lung neoplasms. This enzyme may play a role in lung cancer development and serve as a diagnostic marker.

More Related Videos

Telomerase Activity in the Various Regions of Mouse Brain: Non-Radioactive Telomerase Repeat Amplification Protocol (TRAP) Assay
10:14

Telomerase Activity in the Various Regions of Mouse Brain: Non-Radioactive Telomerase Repeat Amplification Protocol (TRAP) Assay

Published on: September 2, 2014

The Establishment of a Lung Colonization Assay for Circulating Tumor Cell Visualization in Lung Tissues
07:39

The Establishment of a Lung Colonization Assay for Circulating Tumor Cell Visualization in Lung Tissues

Published on: June 16, 2018

Related Experiment Videos

Last Updated: Jun 8, 2026

Semi-quantitative Detection of RNA-dependent RNA Polymerase Activity of Human Telomerase Reverse Transcriptase Protein
08:26

Semi-quantitative Detection of RNA-dependent RNA Polymerase Activity of Human Telomerase Reverse Transcriptase Protein

Published on: June 12, 2018

Telomerase Activity in the Various Regions of Mouse Brain: Non-Radioactive Telomerase Repeat Amplification Protocol (TRAP) Assay
10:14

Telomerase Activity in the Various Regions of Mouse Brain: Non-Radioactive Telomerase Repeat Amplification Protocol (TRAP) Assay

Published on: September 2, 2014

The Establishment of a Lung Colonization Assay for Circulating Tumor Cell Visualization in Lung Tissues
07:39

The Establishment of a Lung Colonization Assay for Circulating Tumor Cell Visualization in Lung Tissues

Published on: June 16, 2018

Area of Science:

  • Oncology
  • Molecular Biology

Context:

  • Lung cancer is a leading cause of cancer-related mortality worldwide.
  • Understanding the molecular mechanisms underlying lung cancer development is crucial for improving diagnosis and treatment.

Purpose:

  • To investigate the correlation between telomerase activity and primary lung cancer.
  • To evaluate telomerase as a potential diagnostic marker for lung cancer.

Summary:

  • Telomerase activity was assessed in primary lung cancer and benign lung neoplasms using the telomeric repeat amplification protocol (TRAP)-PCR-ELISA and TRAP-silver staining methods.
  • Significantly elevated telomerase activity was observed in lung cancer tissues compared to benign neoplasms.
  • Telomerase activity was higher in squamous cell carcinomas than adenocarcinomas and increased with lung cancer stage.

Impact:

  • Telomerase expression is implicated in lung cancer oncogenesis and progression.
  • Telomerase activity shows potential as a valuable tumor marker for lung cancer diagnosis.