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Updated: Jun 8, 2026

Semi-quantitative Detection of RNA-dependent RNA Polymerase Activity of Human Telomerase Reverse Transcriptase Protein
Published on: June 12, 2018
[Telomerase activity in human primary lung caner.]
1Respiratory Department , First Affliated Hospital of Hubei Medical University , Wuhan , Hubei 430060 , P. R. China.
Background:
To investigate the relationship between telomarase activity and primary lung cancer.
Methods:
Telomerase activity was detected in 24 primary lung cancer tissues and 6 benign neoplasms of the lungs by telomeric repeat amplification protocol (TRAP)-PCR-ELISA and TRAP-silver staining.
Results:
The levels of telomerase activity in primary lung cancer tissues were significantly higher than those in benign neoplasms. Positive rate of telomerase activity in lung cancer tissues was 75 %. The level of telomerase activity in squmaous cell carcinomas was significantly higher than that in adenocarcinomas ( t = 2. 235 , P < 0. 05) . The positive rate of telomerase was 50 % in stage II lung cancer and 92. 3 % in stage III disease.
Conclusions:
Telomerase expression might be involved in the oncogenesis and progression of lung cancer and could be used as a tumor marker in the diagnosis of lung cancer.
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