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Published on: May 30, 2016
Structured light sheet fluorescence microscopy based on four beam interference
1Department of Chemie, University of Konstanz, D-78457 Konstanz, Germany.
Abstract:
A 3D structured light sheet microscope using a four-faceted symmetric pyramid is presented. The sample is illuminated by the resulting four beam interference field. This approach combines advantages of standing wave and structured illumination microscopy. Examples of micrographs of fluorescently labeled Chinese hamster ovary (CHO) cells as well as of the compound eyes of drosophila are shown and the optical sectioning ability of our system is demonstrated. The capabilities and the limitations of the scheme are discussed.
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