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Updated: Jun 8, 2026

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy
Published on: May 16, 2022
Imaging through turbid layers by scanning the phase conjugated second harmonic radiation from a nanoparticle
Chia-Lung Hsieh1, Ye Pu, Rachel Grange
1School of Engineering, EPFL, Station 17, 1015 Lausanne, Switzerland. chia-lung.hsieh@epfl.ch
Abstract:
We demonstrate imaging through a turbid layer by using digital phase conjugation of the second harmonic field radiated from a beacon nanoparticle. We show that the phase-conjugated focus can be displaced from its initial position by illuminating the same region of the turbid layer with an angular offset. An image is obtained by scanning the phase-conjugated focus through the turbid layer in a region around the nanoparticle. We obtain a clear image of the target by measuring the light transmitted through it when scanning the focused beam.

