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Related Concept Videos

Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and the...
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview01:13

Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview

Attenuated total reflectance (ATR) infrared spectroscopy is a powerful analytical technique used to study the composition of materials. It is widely employed in chemistry, materials science, forensic science, and other fields where sample characterization is required. ATR has several advantages over traditional transmission IR spectroscopy, including the requirement of little to no sample preparation and the ability to analyze a wide range of samples.
The ATR process begins by directing a beam...

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A Multimodal Wide-Field Fourier-Transform Raman Microscope
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Time-resolved spatially offset Raman spectroscopy for depth analysis of diffusely scattering layers.

Ingeborg E Iping Petterson1, Patrick Dvořák, Joost B Buijs

  • 1Biomolecular Analysis and Spectroscopy, Laser Centre Vrije Universiteit, Amsterdam, The Netherlands.

The Analyst
|October 14, 2010
PubMed
Summary

Advanced Raman spectroscopy techniques, time-resolved (TR) and spatially offset (SORS), successfully retrieved chemical information from materials hidden beneath thick, opaque polymer layers, demonstrating potential for biomedical applications.

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Area of Science:

  • Spectroscopy
  • Materials Science
  • Photonics

Background:

  • Obtaining Raman spectra from subsurface materials is challenging due to light scattering and absorption.
  • Conventional Raman spectroscopy is limited in its ability to probe through opaque or highly scattering media.

Purpose of the Study:

  • To investigate the efficacy of time-resolved (TR) Raman spectroscopy and spatially offset Raman spectroscopy (SORS) for analyzing materials beneath opaque layers.
  • To determine photon migration speeds in various polymers using TR-Raman measurements.
  • To assess the maximum achievable depth for obtaining Raman spectra through scattering materials.

Main Methods:

  • Utilized TR-Raman spectroscopy and SORS with a 460 nm, 3 ps pulsed Ti:sapphire laser.
  • Employed both continuous-wave CCD and time-resolved intensified CCD cameras for detection.
  • Collected Raman photons in backscatter mode with and without lateral spatial offset.
  • Measured signal delay to determine photon migration speeds through polymers like Teflon, polythene, Delrin, and Nylon.

Main Results:

  • Successfully obtained Raman spectra from a second layer of polyethylene terephthalate (PET) through up to 7 mm of Teflon.
  • Quantified photon migration speeds in different polymer samples.
  • Demonstrated improved selectivity for deeper layers by combining TR-Raman and SORS.

Conclusions:

  • TR-Raman and SORS are effective techniques for non-destructively analyzing materials hidden beneath diffusely scattering layers.
  • This approach holds significant promise for in-situ chemical analysis in various fields, particularly biomedical applications.
  • The study provides a foundation for developing advanced spectroscopic methods for subsurface material characterization.