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Updated: Jan 12, 2026

Characterization of Nanocrystal Size Distribution using Raman Spectroscopy with a Multi-particle Phonon Confinement Model
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Characterizing nanoparticle size and composition using microfluidic Raman diffusion-ordered spectroscopy.

Robert W Schmidt1,2, Giulia Giubertoni2, Paul Kolpakov3

  • 1LaserLaB Amsterdam, Department of Physics and Astronomy, Vrije Universiteit Amsterdam, De Boelelaan 1081, 1081 HV Amsterdam, the Netherlands. f.ariese@vu.nl.

Analytical Methods : Advancing Methods and Applications
|October 30, 2025
PubMed
Summary
This summary is machine-generated.

Microfluidic Raman Diffusion-Ordered Spectroscopy (Raman-DOSY) offers a fast method to analyze nanoparticle size and chemical makeup simultaneously. This technique provides crucial data for nanoparticle characterization in solution.

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Area of Science:

  • Analytical Chemistry
  • Materials Science
  • Nanotechnology

Background:

  • Characterizing nanoparticles requires methods to determine both size and chemical composition.
  • Simultaneous analysis of these properties is often challenging with traditional techniques.

Purpose of the Study:

  • To demonstrate the capability of microfluidic Raman Diffusion-Ordered Spectroscopy (Raman-DOSY) for simultaneous nanoparticle size and chemical composition analysis.
  • To establish Raman-DOSY as an efficient method for nanoparticle characterization.

Main Methods:

  • Utilizing a microfluidic channel to separate nanoparticle solution and solvent.
  • Analyzing time-dependent Raman spectra to determine nanoparticle diffusion coefficients and chemical structures.
  • Correlating diffusion coefficients with nanoparticle size.

Main Results:

  • Successfully determined the size and chemical composition of polystyrene nanoparticles (20-100 nm) within one hour.
  • Demonstrated the ability of microfluidic Raman-DOSY to differentiate nanoparticles of varying sizes in mixed samples.
  • Obtained both size and chemical-structure information from a single experiment.

Conclusions:

  • Microfluidic Raman-DOSY is a powerful and rapid technique for combined nanoparticle size and composition analysis.
  • This method shows significant promise for advanced nanoparticle characterization in various applications.
  • The technique offers a streamlined approach compared to conventional multi-step characterization methods.