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High-definition Fourier Transform Infrared (FT-IR) Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology
Published on: January 21, 2015
Multidimensional Infrared Spectroscopy as a Bioanalytical Technique
Sander Woutersen1, Neil T Hunt2
1Van 't Hoff Institute for Molecular Sciences, University of Amsterdam, 1098XH Amsterdam, The Netherlands.
Chemical Reviews
|July 16, 2026
Summary
Two-dimensional infrared (2D-IR) spectroscopy is now a powerful tool for analyzing biomolecules, enabling label-free characterization of protein structure and interactions for applications in drug design and diagnostics.
Area of Science:
- Biophysical Chemistry
- Spectroscopy
- Biomolecular Analysis
Background:
- Two-dimensional infrared (2D-IR) spectroscopy has evolved significantly since the late 20th century.
- Advances in laser technology enable rapid data acquisition with minimal sample under physiological conditions.
Purpose of the Study:
- To review the development of 2D-IR spectroscopy as an analytical tool.
- To discuss current and emerging applications in biomolecular analysis, drug design, and diagnostics.
Main Methods:
- Review of pioneering developments and current state-of-the-art in 2D-IR spectroscopy.
- Discussion of strategies to enhance measurement sensitivity and data quality.
- Exploration of machine learning integration for future development.
Main Results:
- 2D-IR spectroscopy can now characterize label-free protein structure and biomolecular interactions.
- The technique is applicable to complex mixtures and biological tissues.
- Enhanced sensitivity and data quality are extending the reach of 2D-IR.
Conclusions:
- 2D-IR spectroscopy is a rapidly advancing bioanalytical tool with significant potential.
- Emerging methods like machine learning are poised to further enhance its capabilities.
- Challenges and opportunities exist for its widespread adoption in bioanalysis.
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