Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Mutagenicity and Carcinogenicity

Mutagenicity and Carcinogenicity

Mutagenicity and carcinogenicity refer to the ability of drugs to cause genetic defects and induce cancer, respectively. The International Agency for Research on Cancer (IARC) classifies agents into four groups based on their carcinogenic potential. Group 1 agents are known human carcinogens; group 2A agents are probably carcinogenic to humans; group 3 agents lack data to support their role in carcinogenesis; and group 4 includes agents for which data support that they are not likely to be...

In-vitro Mutagenesis

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

Amines to Sulfonamides: The Hinsberg Test

Amines to Sulfonamides: The Hinsberg Test

The Hinsberg test is a method to identify primary, secondary and tertiary amines, named after its pioneer, Oscar Hinsberg. Here, amines are treated with benzenesulfonyl chloride, also known as the Hinsberg reagent, in the presence of an excess of aqueous base, followed by acidification. Based on the nature of the amines, different changes are observed.
Generally, a primary amine reacts with the Hinsberg reagent to produce an N-substituted benzenesulfonamide. The electron-withdrawing sulfonyl...

In vitro Mutagenesis

In vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

[Rare intra-abdominal tumor].

Der Chirurg; Zeitschrift fur alle Gebiete der operativen Medizen·2019

Same author

Derivation of point of departure (PoD) estimates in genetic toxicology studies and their potential applications in risk assessment.

Environmental and molecular mutagenesis·2014

Same author

Cell kinetics, development of stomata and some effects of colchicine in barley.

Planta·2014

Same author

Direct measurement of stomatal cell production in barley.

Planta·2014

Same author

Stomatal development in barley as a bioassay for cell differentiation: Its use with X-rays and gibberellic acid.

Planta·2014

Same author

Structural and functional properties of the coleoptile chloroplast: Photosynthesis and photosensory transduction.

Photosynthesis research·2013

Search research articles

Related Experiment Video

Updated: Jun 8, 2026

Loop-Mediated Isothermal Amplification for Screening Salmonella in Animal Food and Confirming Salmonella from Culture Isolation

Loop-Mediated Isothermal Amplification for Screening Salmonella in Animal Food and Confirming Salmonella from Culture Isolation

Published on: May 20, 2020

The Salmonella (Ames) test for mutagenicity.

E Zeiger¹, K Mortelmans

¹Environmental Toxicology Program/National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina, USA.

Current Protocols in Toxicology

|October 14, 2010

Summary

The Ames test detects DNA-mutating chemicals using Salmonella bacteria. This assay identifies mutagens by observing the dose-dependent increase in his-independent bacterial colonies, crucial for toxicology studies.

More Related Videos

Modified Most Probable Number Assay to Quantify Salmonella in Raw and Ready-to-Cook Chicken Products

Modified Most Probable Number Assay to Quantify Salmonella in Raw and Ready-to-Cook Chicken Products

Published on: January 31, 2025

Quasi-metagenomic Analysis of Salmonella from Food and Environmental Samples

Quasi-metagenomic Analysis of Salmonella from Food and Environmental Samples

Published on: October 25, 2018

Related Experiment Videos

Last Updated: Jun 8, 2026

Loop-Mediated Isothermal Amplification for Screening Salmonella in Animal Food and Confirming Salmonella from Culture Isolation

Loop-Mediated Isothermal Amplification for Screening Salmonella in Animal Food and Confirming Salmonella from Culture Isolation

Published on: May 20, 2020

Modified Most Probable Number Assay to Quantify Salmonella in Raw and Ready-to-Cook Chicken Products

Modified Most Probable Number Assay to Quantify Salmonella in Raw and Ready-to-Cook Chicken Products

Published on: January 31, 2025

Quasi-metagenomic Analysis of Salmonella from Food and Environmental Samples

Quasi-metagenomic Analysis of Salmonella from Food and Environmental Samples

Published on: October 25, 2018

Area of Science:

Microbiology
Toxicology
Genetics

Background:

The Ames test is a standard method for assessing chemical mutagenicity.
It relies on detecting reverse mutations in Salmonella typhimurium strains.
Mutagenic chemicals increase the frequency of his-independent bacterial colonies.

Purpose of the Study:

To provide comprehensive protocols for performing the Ames test.
To detail methods for handling various chemical types and quantities.
To outline procedures for metabolic activation and strain management.

Main Methods:

Basic plate test with and without preincubation.
Protocols for volatile compounds, gases, and limited sample sizes.
Preparation of the S-9 metabolic activation system.

Main Results:

The Ames test quantifies mutagenicity by measuring the reversion frequency.
Results are typically dose-dependent.
The assay is adaptable for diverse chemical properties.

Conclusions:

This unit offers a robust framework for Ames test implementation.
It ensures reliable detection of mutagenic potential across various conditions.
Standardized protocols enhance the accuracy and reproducibility of mutagenicity testing.