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Preparation of Viral DNA from Nucleocapsids
Published on: August 16, 2011
Influence of extraction protocol on physical properties of parvovirus B19 DNA
Bertfried Matz1, Bernd Kupfer, Thomas R Kreil
1Institute of Virology, University of Bonn, Sigmund-Freud-Strasse 25, D-53105 Bonn, Germany.
Journal of Clinical Microbiology
|October 15, 2010
Abstract:
While establishing a quantification standard for parvovirus B19 diagnostics, we extracted viral DNA from a high-titered human plasma by using three commercial kits. Despite similar viral DNA yields being obtained, striking differences in electrophoretic mobilities of the extracted nucleic acids were observed.
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