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Related Experiment Video

Updated: Jun 8, 2026

The Automated Crystallography Pipelines at the EMBL HTX Facility in Grenoble

The Automated Crystallography Pipelines at the EMBL HTX Facility in Grenoble

Published on: June 5, 2021

The JCSG high-throughput structural biology pipeline.

Marc André Elsliger¹, Ashley M Deacon, Adam Godzik

¹Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA, USA.

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications

|October 15, 2010

Summary

The Joint Center for Structural Genomics developed a high-throughput pipeline, producing over 1000 protein structures. This advanced structural biology approach expands coverage of the protein universe and entire organisms.

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Area of Science:

Structural Biology
Genomics
Biophysics

Background:

The National Institutes of Health (NIH) Protein Structure Initiative (PSI) aims to increase structural coverage of proteins.
The Joint Center for Structural Genomics (JCSG) has been a key contributor to this initiative.
Understanding protein structures is crucial for biological and medical research.

Purpose of the Study:

To report on the achievements of the JCSG's high-throughput structural biology pipeline.
To highlight the pipeline's contribution to expanding structural coverage of proteins and organisms.
To showcase technological and methodological advancements in structural biology.

Main Methods:

Utilized a high-throughput pipeline for structure determination.
Employed parallel processing at multiple stages.
Integrated bioinformatics and biophysical analyses for target characterization and optimization.
Adapted the pipeline for diverse protein targets from bacteria to humans.

Main Results:

Delivered over 1000 protein structures to the scientific community.
Made significant progress in achieving structural coverage of an entire organism.
Developed numerous technological and methodological advances.
Created valuable, free-access web-based tools and applications from processed data.

Conclusions:

The JCSG pipeline is highly effective in high-throughput protein structure determination.
The initiative has substantially advanced the goals of the NIH PSI.
Technological innovations have enabled efficient processing of large-scale structural biology data.
Developed resources facilitate broader access to structural biology tools and information.