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Updated: Jun 8, 2026

Inducible and Reversible Dominant-negative (DN) Protein Inhibition
Published on: January 7, 2019
Construction of a dominant negative form of human hypoxia-inducible factor-2α
Yasuki Higashimura1, Ryoichi Yamaji, Naoki Harada
1Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Osaka, Japan.
Abstract:
We constructed a dominant negative form of human hypoxia-inducible factor (HIF)-2α, HIF-2αDoN, which inhibited HIF transcriptional activity induced by hypoxia and by HIF-2α. HIF-2αDoN formed a complex with HIF-1β and interacted with DNA containing hypoxia response elements (HREs). Thus, the complex appears to inhibit the binding of HIF-2 to HREs, and HIF-2αDoN might provide a useful therapeutic tool for HIF-2α-related diseases.
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