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A Toolkit to Enable Hydrocarbon Conversion in Aqueous Environments
Published on: October 2, 2012
Measurement of aryl and alcohol sulfotransferase activity
J J Sheng1, V Sharma, M W Duffel
1University of Iowa, Iowa City, Iowa, USA.
Current Protocols in Toxicology
|October 15, 2010
Abstract:
Two methods are presented for determining the catalytic activity of aryl and alcohol sulfotransferases. The first assay is a simple and rapid procedure that is based on the extraction of a paired ion formed between the product organic sulfate and methylene blue. The second method employs HPLC analysis of the substrate-dependent formation of PAP, an assay that is particularly useful when the sulfuric acid ester product is chemically unstable.

