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BK virus DNA: cleavage map and sequence analysis.

R C Yang, R Wu

    Proceedings of the National Academy of Sciences of the United States of America
    |May 1, 1978
    PubMed
    Summary
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    Researchers mapped the BK virus (MM strain) genome using restriction enzymes, identifying 61 cleavage sites. They found significant homology to simian virus 40 DNA in a region coding for late mRNA leader sequences.

    Area of Science:

    • Molecular Biology
    • Virology
    • Genomics

    Background:

    • BK virus (BKV) is a human polyomavirus with a circular double-stranded DNA genome.
    • Understanding the BKV genome structure is crucial for studying viral replication and pathogenesis.
    • Previous physical mapping of BKV strains provided a foundational understanding of its genetic organization.

    Purpose of the Study:

    • To construct a detailed physical map of the BK virus (MM strain) genome.
    • To identify and locate specific restriction enzyme cleavage sites within the BKV(MM) genome.
    • To investigate potential sequence homology with other related viral genomes.

    Main Methods:

    • Generation of a physical map by analyzing cleavage sites of 11 distinct restriction enzymes.
    • Determination of the precise locations of 61 identified restriction enzyme cutting sites on the BKV(MM) DNA.

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  • Preliminary nucleotide sequencing of the BKV(MM) genome in the 0.70-0.75 map position region.

    • Main Results:

    • A comprehensive physical map of the BK virus (MM strain) genome was successfully established.
    • Sixty-one specific cleavage sites for 11 different restriction enzymes were precisely mapped.
    • An 80% nucleotide sequence homology was observed between BKV(MM) DNA (0.714-0.744 map positions) and simian virus 40 DNA (0.722-0.752 map positions).

    Conclusions:

    • The detailed physical map provides a high-resolution reference for the BK virus (MM strain) genome.
    • The identified homology suggests functional or evolutionary relatedness between BKV and SV40 in specific genomic regions.
    • The homologous region in BKV likely plays a role in the synthesis of late mRNA leader sequences, similar to SV40.