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Updated: Jun 8, 2026

Experimental Measurement of Settling Velocity of Spherical Particles in Unconfined and Confined Surfactant-based Shear Thinning Viscoelastic Fluids
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Experimental Measurement of Settling Velocity of Spherical Particles in Unconfined and Confined Surfactant-based Shear Thinning Viscoelastic Fluids

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A simple apparatus for measuring cell settling velocity.

Zhaowei Wang1, Joanne M Belovich

  • 1Dept. of Chemical and Biomedical Engineering, Cleveland State University, Cleveland, OH 44115, USA. wang_zhaowei@yahoo.com

Biotechnology Progress
|October 15, 2010
PubMed
Summary
This summary is machine-generated.

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Determining cell settling velocity is vital for perfusion culture. A new settling column method accurately measures this, revealing significant variations in hybridoma cells and impacting cell retention strategies.

Area of Science:

  • Biotechnology
  • Bioprocess Engineering
  • Cell Culture Technology

Background:

  • Accurate cell settling velocity is crucial for effective cell retention in perfusion culture systems utilizing gravity settlers.
  • Traditional methods for determining settling velocity can be cumbersome, necessitating simpler and more reliable approaches for bioprocess monitoring.

Purpose of the Study:

  • To develop and validate a simple apparatus, a "settling column," for measuring cell settling velocity.
  • To assess the settling velocities of hybridoma cell lines and nonviable cells.
  • To highlight the importance of routine settling velocity measurements in long-term perfusion cultures.

Main Methods:

  • Development of a "settling column" apparatus for measuring cell settling velocity.
  • Validation of the apparatus using 15-μm polystyrene particles with known physical properties.

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Last Updated: Jun 8, 2026

Experimental Measurement of Settling Velocity of Spherical Particles in Unconfined and Confined Surfactant-based Shear Thinning Viscoelastic Fluids
10:28

Experimental Measurement of Settling Velocity of Spherical Particles in Unconfined and Confined Surfactant-based Shear Thinning Viscoelastic Fluids

Published on: January 3, 2014

Simultaneous Measurement of Turbulence and Particle Kinematics Using Flow Imaging Techniques
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  • Measurement of settling velocities for three hybridoma cell lines at different culture ages and for viable versus nonviable cells.

    • Main Results:

    • The settling column method demonstrated high accuracy, with polystyrene particle settling velocities within 4% of Stokes' law predictions.
    • Significant variations (up to twofold) in settling velocities were observed among different hybridoma cell lines.
    • Settling velocities decreased with increasing cell culture age and were substantially lower (33-50%) for nonviable cells compared to viable cells.

    Conclusions:

    • The developed settling column provides a simple and accurate method for determining cell settling velocity.
    • Cell settling velocity is highly variable across different cell lines, culture phases, and viability states.
    • Routine measurement of cell settling velocity is essential for optimizing cell retention and process control in long-term perfusion cultures.