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Updated: Jun 8, 2026

Horizontal Gel Electrophoresis for Enhanced Detection of Protein-RNA Complexes
Published on: July 28, 2017
Analysis of RNA folding by native polyacrylamide gel electrophoresis
1T.C. Jenkins Department of Biophysics, Johns Hopkins University, Baltimore, Maryland, USA.
Abstract:
Polyacrylamide gel electrophoresis under native conditions (native PAGE) is a well-established and versatile method for probing nucleic acid conformation and nucleic acid-protein interactions. Native PAGE has been used to measure RNA folding equilibria and kinetics under a wide variety of conditions. Advantages of this method are its adaptability, absolute determination of reaction endpoints, and direct analysis of conformational hetereogeneity within a sample. Native PAGE is also useful for resolving ligand-induced structural changes.
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